Perforin-enhancing protein, a low molecular weight protein of cytotoxic lymphocyte granules, enhances perforin lysis
U Winkler, SA Fraser, D Hudig - Biochemical and biophysical research …, 1997 - Elsevier
Perforin is a 68 kD protein found in the granules of cytotoxic lymphocytes and is used by
lymphocytes to form lethal pores in the membranes of the cells they kill. We and others have
found that when perforin is purified, its lytic activity is markedly reduced. ELISAs indicated
that our final recovery of perforin protein was excellent. We decided to determine if depletion
of other granule proteins contributed to the loss of lytic activity. We isolated perforin to the
point where lytic activity was diminished and added back granule proteins that had no lytic …
lymphocytes to form lethal pores in the membranes of the cells they kill. We and others have
found that when perforin is purified, its lytic activity is markedly reduced. ELISAs indicated
that our final recovery of perforin protein was excellent. We decided to determine if depletion
of other granule proteins contributed to the loss of lytic activity. We isolated perforin to the
point where lytic activity was diminished and added back granule proteins that had no lytic …
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